Yet another CRISPR meeting?

Not exactly.

Our aim is to directly empower you to use the CRISPR technology more effectively in your work. Last year, Genome Engineering 3.0 was a great success in allowing us to share our practical experience in person (rather than just online), and Genome Engineering 4.0 follows on that tradition while incorporating some of the most exciting developments in the field.

You will get to hear about recent developments in CRISPR-based genome editing, from improved specificity to novel applications, and have opportunities to meet with experts with hands-on experience in a range of areas. In addition, you'll have a chance to present your own CRISPR work at a poster session and get feedback from colleagues and leading figures in the field.

Come to:
  • learn about the design, implementation, and analysis of CRISPR experiments;
  • discuss latest technical advances and share ideas;
  • troubleshoot your experiments;
  • explore diverse applications of CRISPR nucleases;
  • get answers to your questions, both general and specific.


This workshop is a collaboration between many of the labs developing CRISPR genome engineering.

Keynote speaker:

Sylvain Moineau of Laval University

You will receive practical advice on:
  • Basics of targeted mutagenesis
  • High throughput applications and data analysis
  • Pooled knockout screens
  • Understanding specificity and off target effects
  • Homologous recombination
  • Genome-scale transcriptional control
  • Editing of iPSCs, ESCs, and HSCs
  • Making transgenic mice with Cas9
  • In vivo delivery of Cas9
  • Editing in model and non-model organisms
Confirmed Speakers

  • Rodolphe Barrangou, North Carolina State University
  • Dan Bauer, Harvard
  • Ethan Bier, UC San Diego
  • Connie Cepko, Harvard
  • Jim Collins, MIT
  • Jacob Corn, Innovative Genomics Insitute/UC Berkeley
  • Dan Durocher, University of Toronto
  • Jim Haber, Brandeis University
  • Melissa Harrison, University of Wisconsin
  • Eugene Koonin, NCBI
  • Wenning Qin, Biogen
  • Chris Vakoc, CSHL
  • Dan Voytas, University of Minnesota



Want to keep wearing your winter gear in May? Craving sunny weather that suddenly turns into wintry mix? Come and spend a beautiful spring weekend in Cambridge, MA!

The Broad Institute
415 Main St.
Cambridge, MA 02142

Hosted by the Broad Institute and Aeronaut Brewery



What do I need to do?

Register to attend the workshop

Can I present my work?

You are encouraged to share your work with your fellow workshop attendees. For this purpose we will have an informal poster session on Saturday, May 7. Sign up to present your work via the registration link or email your abstract to by April 11.

Do I need to pay for anything?

Based on the feedback from last year, we will be charging a token fee to hold your spot and cover coffee and snacks. The rest of the event is made possible by the generosity of the Broad Institute, Office of Strategic Alliances and Partnering, BioMed Ventures, Addgene, and Aeronaut Brewery.


Contact Us

If you have any questions, please email us at